Shu, X., Shi, Y., Huang, Y. et al. Transcription tuned by S-nitrosylation underlies a mechanism for Staphylococcus aureus to circumvent vancomycin killing. Nature Communications 14, 2318 (2023).
Journal Nature Communications
doi 10.1038/s41467-023-37949-0 IF(2022) 16.6
Services Polyclonal Antibody Production System Antibody Development
Five microliters of the diluted samples were saved as input sample at ?20 °C, and another 500 μL was incubated with 2 μg of anit-MgrA polyclonal antibody (commercially prepared by MerryBio) or anit-WalR polyclonal antibodyor the corresponding IgG antibodyovernight at 4 °C with gentle rotation.
Zhang S, Zhang Y, Zhang P, et al. Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis. Heliyon. 2023;9(6):e17314. Published 2023 Jun 14.
Journal Nature Communications
doi 10.1016/j.heliyon.2023.e17314 IF(2022) 4
Services Monoclonal Antibody Production System Prokaryotic Expression
A PLXND1-targeted molecular probe was synthesized by conjugation of a single-domain antibody (A12) [22,23] and Cy5 fluorescent dyes (A12-Cy5; MerryBio Co., Ltd., Nanjing, China).
Zou H, Ben T, Wu P, et al. Effective anti-inflammatory phenolic compounds from dandelion: identification and mechanistic insights using UHPLC-ESI-MS/MS, fluorescence quenching and anisotropy, molecular docking and dynamics simulation. Food Science and Human Wellness, 2023, 12(6): 2184-2194.
Journal Food Science and Human Wellness
doi 10.1016/j.fshw.2023.03.031 IF(2022) 7.0
Services Plasmid construction & Protein purification
The coding regions of human MD-2 (1-149) (Gene ID: 23643) and TLR4 (27-631) (Gene ID: 7099) were codon optimized, synthesized, and inserted into the restriction sites, NdeI and HindIII of the pET30a(+) expression vector (MerryBio Co., Ltd).
The final constructs containing a His6-tag at the N-termini of MD-2 and TLR4 were verified by DNA sequencing (MerryBio Co., Ltd.).
The obtained protein was then purified by metal ion affinity chromatography using a Ni-IDA column (45–165 μm) (MerryBio Co., Ltd.) equilibrated with the lysis buffer, washed with a buffer solution (50 mmol/L Tris-HCl, 300 mmol/L NaCl, 8 mol/L Urea, 20 mmol/L imidazole) and eluted with another buffer solution (50 mmol/L Tris-HCl, 300 mmol/L NaCl, 8 mol/L Urea, 500 mmol/L imidazole).

Yu Q, Li C, Niu Q, et al. Hepatic COX1 loss leads to impaired autophagic flux and exacerbates nonalcoholic steatohepatitis. Acta Pharmaceutica Sinica B. 2023;13(6):2628-2644.
Journal Acta Pharmaceutica Sinica B
doi 10.1016/j.apsb.2023.03.008 IF(2022) 14.5
Services Recombinant Protein Production System Prokaryotic Expression
In brief, recombinant mouse COX1 protein (Lot No. P04012021; MerryBio, Nanjing, China) was immobilized via amine coupling on a flow cell of the chip.
Recombinant mouse WIPI2 (Lot No. P30093114; MerryBio, Nanjing, China) was diluted in the running buffer and then injected at different concentrations and passed over adjacent target and control flow cells at a flow rate of 30 μL/min for 180 s, and the decomposition period was adjusted to 300 s.
Zhang H, Zhang C, Wang Z, Cao W, Yu M, Sun Y. Antibody- and aptamer-free SERS substrate for ultrasensitive and anti-interference detection of SARS-CoV-2 spike protein in untreated saliva. Biosensors and Bioelectronics. 2023;237:115457.
Journal Biosensors and Bioelectronics
doi 10.1016/j.bios.2023.115457 IF(2022) 12.6
Services Recombinant Protein Production System Prokaryotic Expression
SARS-CoV-2 spike protein (molecular weight of 70 kDa) was ordered from MerryBio Co. Ltd. (China).
Huang D, Li S, Lu Y, Sha M, Li J. Effects of diflubenzuron on shrimp (Neocaridina palmata) in freshwater systems dominated by submerged plant (Ceratophyllum demersum). Chemosphere. 2023;313:137567.
Journal Chemosphere
doi 10.1016/j.chemosphere.2022.137567 IF(2022) 8.8
Services Polyclonal Antibody Production System Antibody Development
A type of IgG purified from polyclonal antibodies of chitobiase (MerryBio? Co. Ltd., Nanjing, China) was used to test the immune-reactive content of the enzyme in the dilutions.
Hu, P., Ding, H., Liu, H. et al. Regulatory basis for reproductive flexibility in a meningitis-causing fungal pathogen. Nature Communications 13, 7938 (2022).
Journal Nature Communications
doi 10.1038/s41467-022-35549-y IF(2022) 16.6
Services Recombinant Protein Production System Prokaryotic Expression
All proteins were expressed by MerryBio Co.,Ltd.
He J, Zhu YN, Wang B, et al. piRNA-guided intron removal from pre-mRNAs regulates density-dependent reproductive strategy. Cell Reports. 2022;39(4):110593.
Journal Cell Reports
doi 10.1016/j.celrep.2022.110593 IF(2022) 8.8
Services Plasmid construction System Gene Overexpression
The recombinant exon 9-Copia-exon 10 sequence was cloned into the pAc5.1/V5-HisB vector or pSV2 plasmid (Merrybio, China), respectively.